Detailed information of TA system TA096056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 543220..543947
• Replicon: chromosome
• Accession: NZ_CP026803
• Organism: Shigella flexneri strain 89-141

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: C1P88_RS02795
• Protein ID: WP_000547563.1
• Coordinates: 543636..543947 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C1P88_RS02790
• Protein ID: WP_000126296.1
• Coordinates: 543220..543639 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P88_RS02770 (538270)	538270..538797	-	528	WP_001078777.1	electron transport protein HydN	-
C1P88_RS02775 (538946)	538946..539956	-	1011	WP_024260799.1	DNA-binding transcriptional regulator AscG	-
C1P88_RS02780 (540216)	540216..541673	+	1458	WP_001107865.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C1P88_RS02785 (541682)	541682..543105	+	1424	Protein_530	6-phospho-beta-glucosidase AscB	-
C1P88_RS02790 (543220)	543220..543639	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
C1P88_RS02795 (543636)	543636..543947	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
C1P88_RS02800 (544120)	544120..544863	-	744	WP_005076621.1	hypothetical protein	-
C1P88_RS02805 (544906)	544906..545376	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C1P88_RS02810 (545369)	545369..545779	-	411	WP_001291916.1	formate hydrogenlyase assembly protein HycH	-
C1P88_RS02815 (545776)	545776..546543	-	768	Protein_536	formate hydrogenlyase subunit HycG	-
C1P88_RS02820 (546543)	546543..547085	-	543	WP_000955078.1	formate hydrogenlyase subunit HycF	-
C1P88_RS02825 (547095)	547095..548804	-	1710	WP_005076626.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T96056 WP_000547563.1 NZ_CP026803:c543947-543636 [Shigella flexneri]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T96056 NZ_CP026803:c543947-543636 [Shigella flexneri]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT96056 WP_000126296.1 NZ_CP026803:c543639-543220 [Shigella flexneri]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT96056 NZ_CP026803:c543639-543220 [Shigella flexneri]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22