Detailed information of TA system TA096053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4895402..4896129
• Replicon: chromosome
• Accession: NZ_CP026802
• Organism: Shigella sonnei strain ATCC 29930

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: C1P89_RS25205
• Protein ID: WP_000547563.1
• Coordinates: 4895402..4895713 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C1P89_RS25210
• Protein ID: WP_000126296.1
• Coordinates: 4895710..4896129 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P89_RS25175 (4890556)	4890556..4892253	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
C1P89_RS25180 (4892263)	4892263..4892805	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
C1P89_RS25185 (4892805)	4892805..4893572	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
C1P89_RS25190 (4893569)	4893569..4893979	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C1P89_RS25195 (4893972)	4893972..4894442	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C1P89_RS25200 (4894467)	4894467..4895240	+	774	WP_001026444.1	hypothetical protein	-
C1P89_RS25205 (4895402)	4895402..4895713	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
C1P89_RS25210 (4895710)	4895710..4896129	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
C1P89_RS25215 (4896243)	4896243..4897667	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
C1P89_RS25220 (4897676)	4897676..4899133	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C1P89_RS25225 (4899393)	4899393..4900403	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
C1P89_RS25230 (4900551)	4900551..4901078	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T96053 WP_000547563.1 NZ_CP026802:4895402-4895713 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T96053 NZ_CP026802:4895402-4895713 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT96053 WP_000126296.1 NZ_CP026802:4895710-4896129 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT96053 NZ_CP026802:4895710-4896129 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22