Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2714979..2715520 Replicon chromosome
Accession NZ_CP026802
Organism Shigella sonnei strain ATCC 29930

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C1P89_RS14290 Protein ID WP_000615976.1
Coordinates 2715242..2715520 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C1P89_RS14285 Protein ID WP_000729704.1
Coordinates 2714979..2715239 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P89_RS14260 (2710448) 2710448..2712160 + 1713 Protein_2731 flagellar biosynthesis protein FlhA -
C1P89_RS14265 (2712283) 2712283..2712780 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
C1P89_RS14270 (2712851) 2712851..2712979 - 129 Protein_2733 ISAs1 family transposase -
C1P89_RS14275 (2713034) 2713034..2713731 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
C1P89_RS14280 (2714020) 2714020..2714793 - 774 WP_127148796.1 C40 family peptidase -
C1P89_RS14285 (2714979) 2714979..2715239 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C1P89_RS14290 (2715242) 2715242..2715520 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C1P89_RS14295 (2715676) 2715676..2716416 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
C1P89_RS14300 (2716387) 2716387..2717154 - 768 WP_000333380.1 class II glutamine amidotransferase -
C1P89_RS14305 (2717360) 2717360..2717938 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2713407..2726381 12974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T96048 WP_000615976.1 NZ_CP026802:2715242-2715520 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T96048 NZ_CP026802:2715242-2715520 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT96048 WP_000729704.1 NZ_CP026802:2714979-2715239 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT96048 NZ_CP026802:2714979-2715239 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References