Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3355662..3356356 Replicon chromosome
Accession NZ_CP026799
Organism Shigella flexneri strain NCTC 9728

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag C1P82_RS17385 Protein ID WP_001263491.1
Coordinates 3355958..3356356 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag C1P82_RS17380 Protein ID WP_000554759.1
Coordinates 3355662..3355955 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P82_RS17360 3351301..3351798 + 498 WP_000006250.1 transposase -
C1P82_RS17365 3352015..3353727 - 1713 Protein_3325 flagellar biosynthesis protein FlhA -
C1P82_RS17370 3353699..3354484 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
C1P82_RS17375 3354555..3355610 + 1056 WP_001226172.1 DNA polymerase IV -
C1P82_RS17380 3355662..3355955 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C1P82_RS17385 3355958..3356356 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C1P82_RS17390 3356366..3356818 + 453 WP_001059862.1 GNAT family N-acetyltransferase -
C1P82_RS17395 3357064..3358107 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
C1P82_RS17400 3358169..3358783 + 615 Protein_3332 peptide chain release factor H -
C1P82_RS17405 3358840..3360297 - 1458 WP_001293006.1 cytosol nonspecific dipeptidase -
C1P82_RS17410 3360559..3361017 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T96031 WP_001263491.1 NZ_CP026799:3355958-3356356 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T96031 NZ_CP026799:3355958-3356356 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT96031 WP_000554759.1 NZ_CP026799:3355662-3355955 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT96031 NZ_CP026799:3355662-3355955 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References