Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3212637..3213255 Replicon chromosome
Accession NZ_CP026799
Organism Shigella flexneri strain NCTC 9728

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C1P82_RS16665 Protein ID WP_001291435.1
Coordinates 3213037..3213255 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag C1P82_RS16660 Protein ID WP_000344797.1
Coordinates 3212637..3213011 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P82_RS16650 3207726..3208919 + 1194 WP_024260231.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C1P82_RS16655 3208942..3212091 + 3150 WP_001132471.1 multidrug efflux RND transporter permease subunit -
C1P82_RS16660 3212637..3213011 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
C1P82_RS16665 3213037..3213255 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C1P82_RS16670 3213427..3213978 + 552 WP_000102569.1 maltose O-acetyltransferase -
C1P82_RS16675 3214094..3214564 + 471 WP_021554965.1 YlaC family protein -
C1P82_RS16680 3214728..3216278 + 1551 WP_005066536.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C1P82_RS16685 3216320..3216673 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C1P82_RS16695 3217052..3217363 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3217393..3218721 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T96029 WP_001291435.1 NZ_CP026799:3213037-3213255 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T96029 NZ_CP026799:3213037-3213255 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT96029 WP_000344797.1 NZ_CP026799:3212637-3213011 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT96029 NZ_CP026799:3212637-3213011 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References