Detailed information of TA system TA096018

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1618016..1618241
• Replicon: chromosome
• Accession: NZ_CP026799
• Organism: Shigella flexneri strain NCTC 9728

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C1P82_RS08135
• Protein ID: WP_000813254.1
• Coordinates: 1618086..1618241 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1618016..1618074 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P82_RS08095	1613831..1614868	-	1038	WP_005105555.1	tyrosine-type recombinase/integrase	-
C1P82_RS08100	1614868..1615044	-	177	WP_044023995.1	DUF4224 domain-containing protein	-
C1P82_RS08105	1615077..1615774	+	698	Protein_1551	IS1 family transposase	-
C1P82_RS08110	1615906..1616220	-	315	WP_001317924.1	3'-5' exoribonuclease	-
C1P82_RS08115	1616250..1616947	-	698	Protein_1553	IS1 family transposase	-
C1P82_RS08120	1617018..1617434	+	417	WP_005069274.1	hypothetical protein	-
-	1618016..1618074	-	59	-	-	Antitoxin
C1P82_RS08135	1618086..1618241	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
C1P82_RS08140	1618283..1618468	-	186	WP_001302544.1	hypothetical protein	-
C1P82_RS08145	1618409..1618687	+	279	WP_011069426.1	hypothetical protein	-
C1P82_RS08150	1618689..1619747	+	1059	Protein_1558	DUF968 domain-containing protein	-
C1P82_RS08155	1619748..1620113	+	366	WP_000140020.1	RusA family crossover junction endodeoxyribonuclease	-
C1P82_RS08160	1620110..1620797	+	688	Protein_1560	antiterminator	-
C1P82_RS24100	1620827..1620948	+	122	Protein_1561	antiterminator	-
C1P82_RS08185	1621593..1621808	+	216	WP_000839572.1	class II holin family protein	-
C1P82_RS08190	1621907..1622581	+	675	WP_134800537.1	IS66-like element accessory protein TnpA	-
C1P82_RS08195	1622578..1622925	+	348	WP_000631709.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	1607915..1643077	35162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T96018 WP_000813254.1 NZ_CP026799:1618086-1618241 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T96018 NZ_CP026799:1618086-1618241 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT96018 NZ_CP026799:c1618074-1618016 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin