Detailed information of TA system TA096016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 854709..855436
• Replicon: chromosome
• Accession: NZ_CP026799
• Organism: Shigella flexneri strain NCTC 9728

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4P7TJ48
• Locus tag: C1P82_RS04345
• Protein ID: WP_000547561.1
• Coordinates: 855125..855436 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C1P82_RS04340
• Protein ID: WP_000126296.1
• Coordinates: 854709..855128 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P82_RS04320	849758..850285	-	528	WP_001078777.1	electron transport protein HydN	-
C1P82_RS04325	850434..851444	-	1011	WP_005051459.1	DNA-binding transcriptional regulator AscG	-
C1P82_RS04330	851704..853161	+	1458	WP_001107865.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C1P82_RS04335	853170..854594	+	1425	WP_005105741.1	6-phospho-beta-glucosidase AscB	-
C1P82_RS04340	854709..855128	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
C1P82_RS04345	855125..855436	-	312	WP_000547561.1	type II toxin-antitoxin system HigB family toxin	Toxin
C1P82_RS04350	855609..856352	-	744	WP_044024082.1	hypothetical protein	-
C1P82_RS04355	856395..856865	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C1P82_RS04360	856858..857268	-	411	WP_001291916.1	formate hydrogenlyase assembly protein HycH	-
C1P82_RS04365	857265..858032	-	768	WP_000067379.1	formate hydrogenlyase subunit HycG	-
C1P82_RS04370	858032..858574	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
C1P82_RS04375	858584..860293	-	1710	WP_005063855.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12370.06 Da
• Isoelectric Point: 9.7105

>T96016 WP_000547561.1 NZ_CP026799:c855436-855125 [Shigella flexneri]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDAGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T96016 NZ_CP026799:c855436-855125 [Shigella flexneri]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGCAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT96016 WP_000126296.1 NZ_CP026799:c855128-854709 [Shigella flexneri]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT96016 NZ_CP026799:c855128-854709 [Shigella flexneri]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A4P7TJ48