Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2543469..2543971 Replicon chromosome
Accession NZ_CP026795
Organism Shigella boydii strain ATCC BAA-1247

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C5B80_RS13655 Protein ID WP_000767829.1
Coordinates 2543717..2543971 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C5B80_RS13650 Protein ID WP_001259255.1
Coordinates 2543469..2543720 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B80_RS13625 2538647..2539714 - 1068 WP_000080095.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C5B80_RS13630 2539714..2540784 - 1071 WP_000108980.1 histidinol-phosphate transaminase -
C5B80_RS13635 2540781..2542085 - 1305 WP_039060143.1 histidinol dehydrogenase -
C5B80_RS13640 2542091..2542990 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C5B80_RS13645 2543136..2543186 - 51 WP_001364200.1 his operon leader peptide -
C5B80_RS13650 2543469..2543720 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C5B80_RS13655 2543717..2543971 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C5B80_RS13660 2544054..2544878 + 825 WP_000754747.1 SDR family oxidoreductase -
C5B80_RS13665 2544924..2545853 + 930 WP_039059232.1 LysR family transcriptional regulator -
C5B80_RS13670 2546068..2546130 + 63 WP_010723108.1 membrane protein YoeI -
C5B80_RS13675 2546120..2547478 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C5B80_RS13680 2547657..2548617 + 961 Protein_2605 YeeE/YedE family protein -
C5B80_RS13685 2548631..2548858 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T96008 WP_000767829.1 NZ_CP026795:2543717-2543971 [Shigella boydii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T96008 NZ_CP026795:2543717-2543971 [Shigella boydii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT96008 WP_001259255.1 NZ_CP026795:2543469-2543720 [Shigella boydii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT96008 NZ_CP026795:2543469-2543720 [Shigella boydii]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCTAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References