Detailed information of TA system TA096006

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2353908..2354133
• Replicon: chromosome
• Accession: NZ_CP026795
• Organism: Shigella boydii strain ATCC BAA-1247

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C5B80_RS12600
• Protein ID: WP_000813254.1
• Coordinates: 2353908..2354063 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2354075..2354133 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B80_RS12550	2349056..2350212	+	1157	WP_134796775.1	IS3 family transposase	-
C5B80_RS12560	2350256..2350555	-	300	Protein_2392	DUF968 domain-containing protein	-
C5B80_RS12565	2350595..2352133	-	1539	WP_000099170.1	IS66-like element ISEc22 family transposase	-
C5B80_RS12570	2352182..2352529	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
C5B80_RS12575	2352526..2352906	-	381	WP_001333468.1	IS66 family insertion sequence hypothetical protein	-
C5B80_RS12585	2352999..2353460	-	462	Protein_2396	hypothetical protein	-
C5B80_RS12590	2353462..2353740	-	279	WP_000929754.1	hypothetical protein	-
C5B80_RS12600	2353908..2354063	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2354075..2354133	+	59	-	-	Antitoxin
C5B80_RS12615	2354715..2355157	-	443	Protein_2399	hypothetical protein	-
C5B80_RS12620	2355201..2355581	+	381	WP_001333468.1	IS66 family insertion sequence hypothetical protein	-
C5B80_RS12625	2355578..2355925	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
C5B80_RS12630	2355974..2357512	+	1539	WP_134802545.1	IS66-like element ISEc22 family transposase	-
C5B80_RS12635	2357509..2357868	+	360	WP_073692993.1	3'-5' exoribonuclease	-
C5B80_RS12640	2357927..2358130	+	204	WP_000096344.1	DUF4224 domain-containing protein	-
C5B80_RS12645	2358130..2358485	+	356	Protein_2405	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rhs/PAAR / ipaH9.8	2276162..2372784	96622
	-	inside	IScluster/Tn	-	-	2348231..2359686	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T96006 WP_000813254.1 NZ_CP026795:c2354063-2353908 [Shigella boydii]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T96006 NZ_CP026795:c2354063-2353908 [Shigella boydii]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT96006 NZ_CP026795:2354075-2354133 [Shigella boydii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin