Detailed information of TA system TA095977

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4018567..4018792
• Replicon: chromosome
• Accession: NZ_CP026793
• Organism: Shigella flexneri strain 74-1170

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C1P81_RS20310
• Protein ID: WP_000813254.1
• Coordinates: 4018637..4018792 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4018567..4018625 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P81_RS26975	4014565..4014734	+	170	Protein_3891	hypothetical protein	-
C1P81_RS20275	4014880..4015626	+	747	WP_000788996.1	ATP-binding protein	-
C1P81_RS20280	4015641..4016063	+	423	WP_001118168.1	DUF977 family protein	-
C1P81_RS20285	4016121..4016477	+	357	WP_005048249.1	hypothetical protein	-
C1P81_RS20290	4016570..4016788	+	219	WP_000256998.1	DUF4014 family protein	-
C1P81_RS20295	4016790..4017155	+	366	WP_001229298.1	HNH endonuclease signature motif containing protein	-
C1P81_RS20300	4017152..4017817	+	666	WP_000208062.1	hypothetical protein	-
C1P81_RS20305	4017817..4018182	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	4018567..4018625	-	59	-	-	Antitoxin
C1P81_RS20310	4018637..4018792	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
C1P81_RS20325	4020129..4020728	+	600	WP_000940329.1	DUF1367 family protein	-
C1P81_RS20330	4020728..4021018	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
C1P81_RS20335	4021015..4021569	+	555	WP_000640143.1	DUF1133 family protein	-
C1P81_RS20340	4021722..4021895	+	174	WP_000504450.1	hypothetical protein	-
C1P81_RS20345	4021957..4023113	+	1157	WP_094106037.1	IS3-like element IS600 family transposase	-
C1P81_RS20350	4023184..4023666	+	483	WP_000068107.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	4010650..4076038	65388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T95977 WP_000813254.1 NZ_CP026793:4018637-4018792 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T95977 NZ_CP026793:4018637-4018792 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT95977 NZ_CP026793:c4018625-4018567 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin