Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3025079..3025697 Replicon chromosome
Accession NZ_CP026793
Organism Shigella flexneri strain 74-1170

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C1P81_RS15095 Protein ID WP_001291435.1
Coordinates 3025079..3025297 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID -
Locus tag C1P81_RS15100 Protein ID WP_134799864.1
Coordinates 3025323..3025697 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P81_RS15065 (3020971) 3020971..3021282 - 312 WP_000409911.1 MGMT family protein -
C1P81_RS15075 (3021661) 3021661..3022014 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C1P81_RS15080 (3022056) 3022056..3023606 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C1P81_RS15085 (3023770) 3023770..3024240 - 471 WP_000136192.1 YlaC family protein -
C1P81_RS15090 (3024356) 3024356..3024907 - 552 Protein_2897 maltose O-acetyltransferase -
C1P81_RS15095 (3025079) 3025079..3025297 - 219 WP_001291435.1 HHA domain-containing protein Toxin
C1P81_RS15100 (3025323) 3025323..3025697 - 375 WP_134799864.1 Hha toxicity modulator TomB Antitoxin
C1P81_RS15105 (3026243) 3026243..3029392 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
C1P81_RS15110 (3029415) 3029415..3030608 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3019613..3020941 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T95976 WP_001291435.1 NZ_CP026793:c3025297-3025079 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T95976 NZ_CP026793:c3025297-3025079 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14503.34 Da        Isoelectric Point: 4.9091

>AT95976 WP_134799864.1 NZ_CP026793:c3025697-3025323 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDSTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT95976 NZ_CP026793:c3025697-3025323 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACTCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure

References