Detailed information of TA system TA095975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2987916..2988760
• Replicon: chromosome
• Accession: NZ_CP026793
• Organism: Shigella flexneri strain 74-1170

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: C1P81_RS14895
• Protein ID: WP_014532172.1
• Coordinates: 2987916..2988377 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: C1P81_RS14905
• Protein ID: WP_005053053.1
• Coordinates: 2988449..2988760 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P81_RS14880 (2983084)	2983084..2984814	-	1731	WP_000645013.1	hypothetical protein	-
C1P81_RS14885 (2984867)	2984867..2987020	-	2154	WP_005060811.1	SEL1-like repeat protein	-
C1P81_RS14890 (2987171)	2987171..2987868	+	698	WP_227804304.1	IS1 family transposase	-
C1P81_RS14895 (2987916)	2987916..2988377	-	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
C1P81_RS14905 (2988449)	2988449..2988760	-	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
C1P81_RS14910 (2988944)	2988944..2989834	-	891	WP_000971346.1	heme o synthase	-
C1P81_RS14915 (2989846)	2989846..2990175	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C1P81_RS14920 (2990175)	2990175..2990789	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C1P81_RS14925 (2990779)	2990779..2992770	-	1992	Protein_2865	cytochrome o ubiquinol oxidase subunit I	-
C1P81_RS14930 (2992792)	2992792..2993289	-	498	Protein_2866	COX aromatic rich motif-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.004	flank	IS/Tn	-	-	2987365..2987868	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T95975 WP_014532172.1 NZ_CP026793:c2988377-2987916 [Shigella flexneri]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T95975 NZ_CP026793:c2988377-2987916 [Shigella flexneri]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT95975 WP_005053053.1 NZ_CP026793:c2988760-2988449 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT95975 NZ_CP026793:c2988760-2988449 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0