Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2875857..2876551 Replicon chromosome
Accession NZ_CP026793
Organism Shigella flexneri strain 74-1170

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag C1P81_RS14190 Protein ID WP_001263491.1
Coordinates 2876153..2876551 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag C1P81_RS14185 Protein ID WP_000554759.1
Coordinates 2875857..2876150 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P81_RS14165 (2871496) 2871496..2871993 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
C1P81_RS14170 (2872210) 2872210..2873922 - 1713 Protein_2717 flagellar biosynthesis protein FlhA -
C1P81_RS14175 (2873894) 2873894..2874679 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
C1P81_RS14180 (2874750) 2874750..2875805 + 1056 WP_001226172.1 DNA polymerase IV -
C1P81_RS14185 (2875857) 2875857..2876150 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C1P81_RS14190 (2876153) 2876153..2876551 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C1P81_RS14195 (2876561) 2876561..2877013 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
C1P81_RS14200 (2877259) 2877259..2878302 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
C1P81_RS14205 (2878418) 2878418..2878978 + 561 Protein_2724 peptide chain release factor H -
C1P81_RS14210 (2879035) 2879035..2880492 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
C1P81_RS14215 (2880754) 2880754..2881212 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T95974 WP_001263491.1 NZ_CP026793:2876153-2876551 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T95974 NZ_CP026793:2876153-2876551 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT95974 WP_000554759.1 NZ_CP026793:2875857-2876150 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT95974 NZ_CP026793:2875857-2876150 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References