Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1127547..1128274 Replicon chromosome
Accession NZ_CP026793
Organism Shigella flexneri strain 74-1170

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C1P81_RS05545 Protein ID WP_000550189.1
Coordinates 1127960..1128274 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C1P81_RS05540 Protein ID WP_000560266.1
Coordinates 1127547..1127963 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P81_RS05530 (1122907) 1122907..1125258 + 2352 WP_000695506.1 alpha-glucosidase -
C1P81_RS05535 (1125484) 1125484..1127502 + 2019 WP_000121487.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
C1P81_RS05540 (1127547) 1127547..1127963 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C1P81_RS05545 (1127960) 1127960..1128274 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C1P81_RS05550 (1128559) 1128559..1129695 - 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C1P81_RS05555 (1129780) 1129780..1130283 + 504 WP_005050988.1 M48 family metallopeptidase -
C1P81_RS05560 (1130360) 1130360..1131052 + 693 WP_000942538.1 vancomycin high temperature exclusion protein -
C1P81_RS05565 (1131131) 1131131..1132129 + 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T95971 WP_000550189.1 NZ_CP026793:c1128274-1127960 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T95971 NZ_CP026793:c1128274-1127960 [Shigella flexneri]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT95971 WP_000560266.1 NZ_CP026793:c1127963-1127547 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT95971 NZ_CP026793:c1127963-1127547 [Shigella flexneri]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References