Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3833224..3833918 Replicon chromosome
Accession NZ_CP026792
Organism Shigella flexneri strain 61-4982

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag C1P80_RS20245 Protein ID WP_001263491.1
Coordinates 3833224..3833622 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag C1P80_RS20250 Protein ID WP_000554759.1
Coordinates 3833625..3833918 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P80_RS20220 3828563..3829021 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
C1P80_RS20225 3829283..3830740 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
C1P80_RS20230 3830797..3831411 - 615 Protein_3768 peptide chain release factor H -
C1P80_RS20235 3831473..3832516 - 1044 WP_005060486.1 RNA ligase RtcB family protein -
C1P80_RS20240 3832762..3833214 - 453 WP_001059861.1 GNAT family N-acetyltransferase -
C1P80_RS20245 3833224..3833622 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C1P80_RS20250 3833625..3833918 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C1P80_RS20255 3833970..3835024 - 1055 Protein_3773 DNA polymerase IV -
C1P80_RS20260 3835095..3835880 - 786 WP_000207561.1 putative lateral flagellar export/assembly protein LafU -
C1P80_RS20265 3835852..3837564 + 1713 Protein_3775 flagellar biosynthesis protein FlhA -
C1P80_RS20270 3837781..3838278 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB 3777388..3839008 61620


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T95968 WP_001263491.1 NZ_CP026792:c3833622-3833224 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T95968 NZ_CP026792:c3833622-3833224 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT95968 WP_000554759.1 NZ_CP026792:c3833918-3833625 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT95968 NZ_CP026792:c3833918-3833625 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References