Detailed information of TA system TA095965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3364968..3365529
• Replicon: chromosome
• Accession: NZ_CP026792
• Organism: Shigella flexneri strain 61-4982

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C1P80_RS17705
• Protein ID: WP_001202685.1
• Coordinates: 3364968..3365231 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: C1P80_RS17710
• Protein ID: WP_000147081.1
• Coordinates: 3365221..3365529 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P80_RS17670	3360069..3360269	-	201	WP_000111769.1	protein ninH	-
C1P80_RS17675	3360364..3360621	-	258	WP_001064799.1	hypothetical protein	-
C1P80_RS17680	3360618..3362009	-	1392	WP_000211324.1	replicative DNA helicase	-
C1P80_RS17685	3362006..3362884	-	879	WP_000988183.1	ATP-binding protein	-
C1P80_RS17690	3362895..3363803	-	909	WP_001247840.1	hypothetical protein	-
C1P80_RS17695	3363790..3364023	-	234	WP_000618004.1	hypothetical protein	-
C1P80_RS17700	3364020..3365036	-	1017	Protein_3288	Rha family transcriptional regulator	-
C1P80_RS17705	3364968..3365231	+	264	WP_001202685.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1P80_RS17710	3365221..3365529	+	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
C1P80_RS17715	3365571..3365747	-	177	WP_024260406.1	NinE family protein	-
C1P80_RS17720	3365744..3366154	-	411	WP_000814621.1	recombination protein NinB	-
C1P80_RS17725	3366151..3366429	-	279	WP_005107095.1	hypothetical protein	-
C1P80_RS17730	3366504..3367328	-	825	WP_075332552.1	hypothetical protein	-
C1P80_RS17740	3368847..3368960	-	114	Protein_3296	DUF1133 family protein	-
C1P80_RS17745	3368957..3369247	-	291	WP_000228005.1	DUF1364 domain-containing protein	-
C1P80_RS17750	3369247..3369480	-	234	Protein_3298	DUF1367 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3329401..3389268	59867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-81)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10105.81 Da
• Isoelectric Point: 10.7080

>T95965 WP_001202685.1 NZ_CP026792:3364968-3365231 [Shigella flexneri]
MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRM
KEVKHDW

Nucleotide

• Length: 264 bp

>T95965 NZ_CP026792:3364968-3365231 [Shigella flexneri]
ATGCGTGCCAGATACCAGCATCTTGTTGAACGCATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTT
TGGTGACGGGCTTTTCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAAC
GCATCATCATGCTGCATAGTTTTGTAAAGAAAGCGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATG
AAGGAGGTTAAGCATGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT95965 WP_000147081.1 NZ_CP026792:3365221-3365529 [Shigella flexneri]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT95965 NZ_CP026792:3365221-3365529 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P7TJC1