Detailed information of TA system TA095963

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2724921..2725146
• Replicon: chromosome
• Accession: NZ_CP026792
• Organism: Shigella flexneri strain 61-4982

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C1P80_RS14370
• Protein ID: WP_000813254.1
• Coordinates: 2724921..2725076 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2725088..2725146 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P80_RS14330	2720047..2720553	-	507	WP_045178261.1	ORF6N domain-containing protein	-
C1P80_RS14340	2721818..2722003	-	186	WP_005049799.1	hypothetical protein	-
C1P80_RS14345	2722144..2722698	-	555	WP_000640143.1	DUF1133 family protein	-
C1P80_RS14350	2722695..2722985	-	291	WP_000228038.1	DUF1364 domain-containing protein	-
C1P80_RS14355	2722985..2723584	-	600	WP_000940329.1	DUF1367 family protein	-
C1P80_RS14360	2723718..2724415	+	698	Protein_2644	IS1 family transposase	-
C1P80_RS14370	2724921..2725076	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2725088..2725146	+	59	-	-	Antitoxin
C1P80_RS14375	2725531..2725896	-	366	WP_000610655.1	DUF551 domain-containing protein	-
C1P80_RS14380	2725896..2726561	-	666	WP_000208062.1	hypothetical protein	-
C1P80_RS14385	2726558..2726923	-	366	WP_001229298.1	HNH endonuclease	-
C1P80_RS14390	2726925..2727143	-	219	WP_000256998.1	DUF4014 family protein	-
C1P80_RS14395	2727236..2727592	-	357	WP_005048249.1	hypothetical protein	-
C1P80_RS14400	2727650..2728072	-	423	WP_001118167.1	DUF977 family protein	-
C1P80_RS14405	2728087..2728833	-	747	WP_000788996.1	ATP-binding protein	-
C1P80_RS24510	2729326..2729580	-	255	Protein_2653	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	2688631..2730732	42101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T95963 WP_000813254.1 NZ_CP026792:c2725076-2724921 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T95963 NZ_CP026792:c2725076-2724921 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT95963 NZ_CP026792:2725088-2725146 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin