Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3728835..3729562 Replicon chromosome
Accession NZ_CP026788
Organism Shigella flexneri 2a strain ATCC 29903

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C1P79_RS19230 Protein ID WP_000550189.1
Coordinates 3728835..3729149 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C1P79_RS19235 Protein ID WP_000560266.1
Coordinates 3729146..3729562 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P79_RS19210 (3724980) 3724980..3725978 - 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -
C1P79_RS19215 (3726057) 3726057..3726749 - 693 WP_000942538.1 vancomycin high temperature exclusion protein -
C1P79_RS19220 (3726826) 3726826..3727329 - 504 WP_005050988.1 M48 family metallopeptidase -
C1P79_RS19225 (3727414) 3727414..3728550 + 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C1P79_RS19230 (3728835) 3728835..3729149 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C1P79_RS19235 (3729146) 3729146..3729562 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C1P79_RS19240 (3729607) 3729607..3731625 - 2019 WP_000121487.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
C1P79_RS19245 (3731851) 3731851..3734202 - 2352 WP_000695506.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T95948 WP_000550189.1 NZ_CP026788:3728835-3729149 [Shigella flexneri 2a]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T95948 NZ_CP026788:3728835-3729149 [Shigella flexneri 2a]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT95948 WP_000560266.1 NZ_CP026788:3729146-3729562 [Shigella flexneri 2a]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT95948 NZ_CP026788:3729146-3729562 [Shigella flexneri 2a]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References