Detailed information of TA system TA095945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1961309..1962153
• Replicon: chromosome
• Accession: NZ_CP026788
• Organism: Shigella flexneri 2a strain ATCC 29903

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: C1P79_RS10535
• Protein ID: WP_014532172.1
• Coordinates: 1961692..1962153 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: C1P79_RS10525
• Protein ID: WP_005053053.1
• Coordinates: 1961309..1961620 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P79_RS10500 (1956780)	1956780..1957277	+	498	Protein_1997	COX aromatic rich motif-containing protein	-
C1P79_RS10505 (1957299)	1957299..1959290	+	1992	Protein_1998	cytochrome o ubiquinol oxidase subunit I	-
C1P79_RS10510 (1959280)	1959280..1959894	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
C1P79_RS10515 (1959894)	1959894..1960223	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
C1P79_RS10520 (1960235)	1960235..1961125	+	891	WP_000971346.1	heme o synthase	-
C1P79_RS10525 (1961309)	1961309..1961620	+	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
C1P79_RS10535 (1961692)	1961692..1962153	+	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
C1P79_RS10545 (1963049)	1963049..1965202	+	2154	WP_005060811.1	SEL1-like repeat protein	-
C1P79_RS10550 (1965255)	1965255..1966985	+	1731	WP_000645013.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.004	flank	IS/Tn	-	-	1962201..1962704	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T95945 WP_014532172.1 NZ_CP026788:1961692-1962153 [Shigella flexneri 2a]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T95945 NZ_CP026788:1961692-1962153 [Shigella flexneri 2a]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTATTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT95945 WP_005053053.1 NZ_CP026788:1961309-1961620 [Shigella flexneri 2a]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT95945 NZ_CP026788:1961309-1961620 [Shigella flexneri 2a]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0