Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1924353..1924971 Replicon chromosome
Accession NZ_CP026788
Organism Shigella flexneri 2a strain ATCC 29903

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C1P79_RS10335 Protein ID WP_001291435.1
Coordinates 1924753..1924971 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag C1P79_RS10330 Protein ID WP_000344797.1
Coordinates 1924353..1924727 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P79_RS10320 (1919442) 1919442..1920635 + 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C1P79_RS10325 (1920658) 1920658..1923807 + 3150 WP_001132484.1 efflux RND transporter permease AcrB -
C1P79_RS10330 (1924353) 1924353..1924727 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
C1P79_RS10335 (1924753) 1924753..1924971 + 219 WP_001291435.1 HHA domain-containing protein Toxin
C1P79_RS10340 (1925143) 1925143..1925694 + 552 Protein_1966 maltose O-acetyltransferase -
C1P79_RS10345 (1925810) 1925810..1926280 + 471 WP_000136192.1 YlaC family protein -
C1P79_RS10350 (1926444) 1926444..1927994 + 1551 WP_050197660.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C1P79_RS10355 (1928036) 1928036..1928389 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C1P79_RS10365 (1928768) 1928768..1929079 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1929109..1930437 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T95944 WP_001291435.1 NZ_CP026788:1924753-1924971 [Shigella flexneri 2a]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T95944 NZ_CP026788:1924753-1924971 [Shigella flexneri 2a]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT95944 WP_000344797.1 NZ_CP026788:1924353-1924727 [Shigella flexneri 2a]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT95944 NZ_CP026788:1924353-1924727 [Shigella flexneri 2a]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References