Detailed information of TA system TA095926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3818801..3819428
• Replicon: chromosome
• Accession: NZ_CP026786
• Organism: Shigella dysenteriae strain NCTC 9718

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A828BKW8
• Locus tag: C1P65_RS20860
• Protein ID: WP_024258354.1
• Coordinates: 3819099..3819428 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: C1P65_RS20855
• Protein ID: WP_000147081.1
• Coordinates: 3818801..3819109 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P65_RS20825	3814798..3815139	+	342	Protein_3886	DUF2138 family protein	-
C1P65_RS20830	3815136..3815696	+	561	WP_001215754.1	DUF1175 domain-containing protein	-
C1P65_RS20835	3815754..3816758	-	1005	Protein_3888	tyrosine-type recombinase/integrase	-
C1P65_RS20840	3816892..3818123	+	1232	WP_134802077.1	IS3-like element IS600 family transposase	-
C1P65_RS20845	3818176..3818586	+	411	WP_000814621.1	recombination protein NinB	-
C1P65_RS20850	3818583..3818804	+	222	WP_001254231.1	NinE family protein	-
C1P65_RS20855	3818801..3819109	-	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
C1P65_RS20860	3819099..3819428	-	330	WP_024258354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1P65_RS20865	3819749..3820426	+	678	WP_000957540.1	phage antirepressor Ant	-
C1P65_RS20870	3820501..3821223	+	723	WP_001004003.1	DNA-binding protein	-
C1P65_RS20875	3821261..3821401	+	141	Protein_3896	HNH endonuclease	-
C1P65_RS20885	3821458..3822152	+	695	WP_134801589.1	IS1 family transposase	-
C1P65_RS20890	3822168..3822488	-	321	Protein_3898	ABC transporter substrate-binding protein ArtJ	-
C1P65_RS20895	3822779..3823447	-	669	WP_000464490.1	arginine ABC transporter permease ArtM	-
C1P65_RS20900	3823447..3824163	-	717	WP_001001693.1	arginine ABC transporter permease ArtQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3811935..3833166	21231
	-	inside	IScluster/Tn	-	-	3816892..3829315	12423

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12837.87 Da
• Isoelectric Point: 9.9966

>T95926 WP_024258354.1 NZ_CP026786:c3819428-3819099 [Shigella dysenteriae]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 330 bp

>T95926 NZ_CP026786:c3819428-3819099 [Shigella dysenteriae]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT95926 WP_000147081.1 NZ_CP026786:c3819109-3818801 [Shigella dysenteriae]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT95926 NZ_CP026786:c3819109-3818801 [Shigella dysenteriae]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A828BKW8

Antitoxin

• AlphaFold DB: A0A4P7TJC1