Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1395543..1396084 Replicon chromosome
Accession NZ_CP026786
Organism Shigella dysenteriae strain NCTC 9718

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag C1P65_RS07930 Protein ID WP_000615980.1
Coordinates 1395806..1396084 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag C1P65_RS07925 Protein ID WP_000729709.1
Coordinates 1395543..1395803 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P65_RS07885 1390802..1391498 + 697 Protein_1473 IS1-like element IS1N family transposase -
C1P65_RS07890 1391512..1391724 + 213 Protein_1474 helix-turn-helix transcriptional regulator -
C1P65_RS07900 1392013..1392710 - 698 Protein_1475 IS1 family transposase -
C1P65_RS07905 1392765..1393109 - 345 Protein_1476 fimbria/pilus periplasmic chaperone -
C1P65_RS07915 1394091..1394408 - 318 Protein_1478 transposase -
C1P65_RS07920 1394584..1395333 - 750 WP_011378552.1 C40 family peptidase -
C1P65_RS07925 1395543..1395803 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C1P65_RS07930 1395806..1396084 + 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C1P65_RS07935 1396240..1396980 + 741 WP_001225679.1 murein L,D-transpeptidase -
C1P65_RS07940 1396951..1397718 - 768 WP_000333379.1 class II glutamine amidotransferase -
C1P65_RS07945 1397924..1398502 - 579 WP_005020694.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fimC / gmhA/lpcA 1355001..1423564 68563


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T95920 WP_000615980.1 NZ_CP026786:1395806-1396084 [Shigella dysenteriae]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T95920 NZ_CP026786:1395806-1396084 [Shigella dysenteriae]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAGG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT95920 WP_000729709.1 NZ_CP026786:1395543-1395803 [Shigella dysenteriae]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT95920 NZ_CP026786:1395543-1395803 [Shigella dysenteriae]
ATGGCTGCAAATGCATTTGTTCGTGCCCGAATCGATGAGGATTTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References