Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3994708..3995249 Replicon chromosome
Accession NZ_CP026784
Organism Shigella dysenteriae strain 80-547

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag C5B79_RS21910 Protein ID WP_000615980.1
Coordinates 3994971..3995249 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag C5B79_RS21905 Protein ID WP_000729709.1
Coordinates 3994708..3994968 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5B79_RS21865 3989967..3990663 + 697 Protein_4085 IS1-like element IS1N family transposase -
C5B79_RS21870 3990677..3990889 + 213 Protein_4086 helix-turn-helix transcriptional regulator -
C5B79_RS21880 3991178..3991875 - 698 Protein_4087 IS1 family transposase -
C5B79_RS21885 3991930..3992274 - 345 Protein_4088 fimbria/pilus periplasmic chaperone -
C5B79_RS21895 3993256..3993573 - 318 Protein_4090 transposase -
C5B79_RS21900 3993749..3994498 - 750 WP_011378552.1 C40 family peptidase -
C5B79_RS21905 3994708..3994968 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C5B79_RS21910 3994971..3995249 + 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C5B79_RS21915 3995405..3996145 + 741 WP_001225679.1 murein L,D-transpeptidase -
C5B79_RS21920 3996116..3996883 - 768 WP_000333379.1 class II glutamine amidotransferase -
C5B79_RS21925 3997089..3997667 - 579 WP_005020694.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fimC / gmhA/lpcA / IlpA 3988918..4025432 36514


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T95904 WP_000615980.1 NZ_CP026784:3994971-3995249 [Shigella dysenteriae]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T95904 NZ_CP026784:3994971-3995249 [Shigella dysenteriae]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAGG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT95904 WP_000729709.1 NZ_CP026784:3994708-3994968 [Shigella dysenteriae]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT95904 NZ_CP026784:3994708-3994968 [Shigella dysenteriae]
ATGGCTGCAAATGCATTTGTTCGTGCCCGAATCGATGAGGATTTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References