Detailed information of TA system TA095893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2032366..2032993
• Replicon: chromosome
• Accession: NZ_CP026784
• Organism: Shigella dysenteriae strain 80-547

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A828BKW8
• Locus tag: C5B79_RS10950
• Protein ID: WP_024258354.1
• Coordinates: 2032664..2032993 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: C5B79_RS10945
• Protein ID: WP_000147081.1
• Coordinates: 2032366..2032674 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B79_RS10915	2028363..2028704	+	342	Protein_2033	DUF2138 family protein	-
C5B79_RS10920	2028701..2029261	+	561	WP_001215754.1	DUF1175 domain-containing protein	-
C5B79_RS10925	2029319..2030323	-	1005	Protein_2035	tyrosine-type recombinase/integrase	-
C5B79_RS10930	2030457..2031688	+	1232	WP_134802077.1	IS3-like element IS600 family transposase	-
C5B79_RS10935	2031741..2032151	+	411	WP_000814621.1	recombination protein NinB	-
C5B79_RS10940	2032148..2032369	+	222	WP_001254231.1	NinE family protein	-
C5B79_RS10945	2032366..2032674	-	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
C5B79_RS10950	2032664..2032993	-	330	WP_024258354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C5B79_RS10955	2033314..2033991	+	678	WP_000957540.1	phage antirepressor Ant	-
C5B79_RS10960	2034066..2034788	+	723	WP_134802076.1	DNA-binding protein	-
C5B79_RS10965	2034826..2034966	+	141	Protein_2043	HNH endonuclease	-
C5B79_RS10975	2035023..2035716	+	694	Protein_2044	IS1 family transposase	-
C5B79_RS10980	2035732..2036052	-	321	Protein_2045	ABC transporter substrate-binding protein ArtJ	-
C5B79_RS10985	2036343..2037011	-	669	WP_000464490.1	arginine ABC transporter permease ArtM	-
C5B79_RS10990	2037011..2037727	-	717	WP_001001693.1	arginine ABC transporter permease ArtQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2025500..2045954	20454
	-	inside	IScluster/Tn	-	-	2030457..2042880	12423

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12837.87 Da
• Isoelectric Point: 9.9966

>T95893 WP_024258354.1 NZ_CP026784:c2032993-2032664 [Shigella dysenteriae]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 330 bp

>T95893 NZ_CP026784:c2032993-2032664 [Shigella dysenteriae]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT95893 WP_000147081.1 NZ_CP026784:c2032674-2032366 [Shigella dysenteriae]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT95893 NZ_CP026784:c2032674-2032366 [Shigella dysenteriae]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A828BKW8

Antitoxin

• AlphaFold DB: A0A4P7TJC1