Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4192467..4193094 Replicon chromosome
Accession NZ_CP026782
Organism Shigella dysenteriae strain 08-3380

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag C1P64_RS23810 Protein ID WP_024258354.1
Coordinates 4192765..4193094 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag C1P64_RS23805 Protein ID WP_000147081.1
Coordinates 4192467..4192775 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P64_RS23780 4188307..4189311 - 1005 WP_000846240.1 ADP-ribosylglycohydrolase family protein -
C1P64_RS23785 4189308..4190519 - 1212 WP_141106345.1 nucleoside permease -
C1P64_RS23790 4190558..4191789 + 1232 WP_134802077.1 IS3-like element IS600 family transposase -
C1P64_RS23795 4191842..4192252 + 411 WP_000814621.1 recombination protein NinB -
C1P64_RS23800 4192249..4192470 + 222 WP_001254231.1 NinE family protein -
C1P64_RS23805 4192467..4192775 - 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
C1P64_RS23810 4192765..4193094 - 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1P64_RS23815 4193415..4194092 + 678 WP_000957540.1 phage antirepressor Ant -
C1P64_RS23820 4194167..4194862 + 696 WP_141106346.1 DNA-binding protein -
C1P64_RS23830 4196196..4196336 + 141 Protein_4318 HNH endonuclease -
C1P64_RS23840 4196393..4197087 + 695 WP_134801589.1 IS1 family transposase -
C1P64_RS23845 4197103..4197423 - 321 Protein_4320 ABC transporter substrate-binding protein ArtJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4174801..4207326 32525
- inside IScluster/Tn - - 4190558..4204252 13694


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T95883 WP_024258354.1 NZ_CP026782:c4193094-4192765 [Shigella dysenteriae]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T95883 NZ_CP026782:c4193094-4192765 [Shigella dysenteriae]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT95883 WP_000147081.1 NZ_CP026782:c4192775-4192467 [Shigella dysenteriae]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT95883 NZ_CP026782:c4192775-4192467 [Shigella dysenteriae]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References