Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2446238..2446779 Replicon chromosome
Accession NZ_CP026782
Organism Shigella dysenteriae strain 08-3380

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag C1P64_RS13455 Protein ID WP_000615980.1
Coordinates 2446238..2446516 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag C1P64_RS13460 Protein ID WP_000729709.1
Coordinates 2446519..2446779 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P64_RS13440 2443820..2444398 + 579 WP_005020694.1 D-sedoheptulose 7-phosphate isomerase -
C1P64_RS13445 2444604..2445371 + 768 WP_000333379.1 class II glutamine amidotransferase -
C1P64_RS13450 2445342..2446082 - 741 WP_001225679.1 murein L,D-transpeptidase -
C1P64_RS13455 2446238..2446516 - 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C1P64_RS13460 2446519..2446779 - 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C1P64_RS13465 2446989..2447738 + 750 WP_011378552.1 C40 family peptidase -
C1P64_RS13470 2447914..2448231 + 318 Protein_2456 transposase -
C1P64_RS13480 2448288..2448982 + 695 WP_134801543.1 IS1 family transposase -
C1P64_RS13485 2449213..2449557 + 345 Protein_2458 fimbria/pilus periplasmic chaperone -
C1P64_RS13490 2449612..2450309 + 698 Protein_2459 IS1 family transposase -
C1P64_RS13500 2450598..2450810 - 213 Protein_2460 helix-turn-helix transcriptional regulator -
C1P64_RS13505 2450824..2451520 - 697 Protein_2461 IS1-like element IS1N family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / fimC 2427097..2452569 25472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T95872 WP_000615980.1 NZ_CP026782:c2446516-2446238 [Shigella dysenteriae]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T95872 NZ_CP026782:c2446516-2446238 [Shigella dysenteriae]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAGG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT95872 WP_000729709.1 NZ_CP026782:c2446779-2446519 [Shigella dysenteriae]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT95872 NZ_CP026782:c2446779-2446519 [Shigella dysenteriae]
ATGGCTGCAAATGCATTTGTTCGTGCCCGAATCGATGAGGATTTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References