Detailed information of TA system TA095859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2054045..2054672
• Replicon: chromosome
• Accession: NZ_CP026780
• Organism: Shigella dysenteriae strain 53-3937

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A828BKW8
• Locus tag: C1P63_RS11525
• Protein ID: WP_024258354.1
• Coordinates: 2054045..2054374 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: C1P63_RS11530
• Protein ID: WP_000147081.1
• Coordinates: 2054364..2054672 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P63_RS11485	2049311..2050027	+	717	WP_001001693.1	arginine ABC transporter permease ArtQ	-
C1P63_RS11490	2050027..2050695	+	669	WP_000464490.1	arginine ABC transporter permease ArtM	-
C1P63_RS11495	2050986..2051306	+	321	Protein_2137	ABC transporter substrate-binding protein ArtJ	-
C1P63_RS11500	2051322..2052015	-	694	Protein_2138	IS1 family transposase	-
C1P63_RS11510	2052072..2052212	-	141	Protein_2139	HNH endonuclease	-
C1P63_RS11515	2052250..2052972	-	723	WP_001004003.1	DNA-binding protein	-
C1P63_RS11520	2053047..2053724	-	678	WP_000957540.1	phage antirepressor Ant	-
C1P63_RS11525	2054045..2054374	+	330	WP_024258354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1P63_RS11530	2054364..2054672	+	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
C1P63_RS11535	2054669..2054890	-	222	WP_001254231.1	NinE family protein	-
C1P63_RS11540	2054887..2055297	-	411	WP_000814621.1	recombination protein NinB	-
C1P63_RS11545	2055350..2056045	-	696	Protein_2146	IS3-like element IS600 family transposase	-
C1P63_RS11550	2056106..2056650	+	545	Protein_2147	transposase	-
C1P63_RS11560	2057983..2058987	+	1005	Protein_2149	tyrosine-type recombinase/integrase	-
C1P63_RS11565	2059045..2059605	-	561	WP_001215754.1	DUF1175 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2041084..2093676	52592
	-	inside	IScluster/Tn	-	-	2044158..2057850	13692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12837.87 Da
• Isoelectric Point: 9.9966

>T95859 WP_024258354.1 NZ_CP026780:2054045-2054374 [Shigella dysenteriae]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 330 bp

>T95859 NZ_CP026780:2054045-2054374 [Shigella dysenteriae]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT95859 WP_000147081.1 NZ_CP026780:2054364-2054672 [Shigella dysenteriae]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT95859 NZ_CP026780:2054364-2054672 [Shigella dysenteriae]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A828BKW8

Antitoxin

• AlphaFold DB: A0A4P7TJC1