Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2016454..2017148 Replicon chromosome
Accession NZ_CP026776
Organism Shigella flexneri strain 98-3193

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag C1P84_RS10865 Protein ID WP_001263491.1
Coordinates 2016750..2017148 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag C1P84_RS10860 Protein ID WP_000554759.1
Coordinates 2016454..2016747 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P84_RS10840 (2012094) 2012094..2012591 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
C1P84_RS10845 (2012808) 2012808..2014520 - 1713 Protein_2066 flagellar biosynthesis protein FlhA -
C1P84_RS10850 (2014492) 2014492..2015277 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
C1P84_RS10855 (2015399) 2015399..2016402 + 1004 Protein_2068 DNA polymerase IV -
C1P84_RS10860 (2016454) 2016454..2016747 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C1P84_RS10865 (2016750) 2016750..2017148 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C1P84_RS10870 (2017158) 2017158..2017610 + 453 WP_001059846.1 GNAT family N-acetyltransferase -
C1P84_RS10875 (2017856) 2017856..2018899 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
C1P84_RS10880 (2019015) 2019015..2019575 + 561 Protein_2073 peptide chain release factor H -
C1P84_RS10885 (2019632) 2019632..2021088 - 1457 Protein_2074 cytosol nonspecific dipeptidase -
C1P84_RS10890 (2021350) 2021350..2021808 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T95821 WP_001263491.1 NZ_CP026776:2016750-2017148 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T95821 NZ_CP026776:2016750-2017148 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT95821 WP_000554759.1 NZ_CP026776:2016454-2016747 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT95821 NZ_CP026776:2016454-2016747 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References