Detailed information of TA system TA095817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1635356..1635884
• Replicon: chromosome
• Accession: NZ_CP026776
• Organism: Shigella flexneri strain 98-3193

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C1P84_RS08890
• Protein ID: WP_005061113.1
• Coordinates: 1635356..1635586 (+)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: C1P84_RS08895
• Protein ID: WP_000147081.1
• Coordinates: 1635576..1635884 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P84_RS08855 (1630424)	1630424..1630624	-	201	WP_000111769.1	protein ninH	-
C1P84_RS08860 (1630719)	1630719..1630976	-	258	WP_001064799.1	hypothetical protein	-
C1P84_RS08865 (1630973)	1630973..1632364	-	1392	WP_000211324.1	replicative DNA helicase	-
C1P84_RS08870 (1632361)	1632361..1633239	-	879	WP_000988183.1	ATP-binding protein	-
C1P84_RS08875 (1633250)	1633250..1634158	-	909	WP_001247840.1	hypothetical protein	-
C1P84_RS08880 (1634145)	1634145..1634378	-	234	WP_000618004.1	hypothetical protein	-
C1P84_RS23725 (1634375)	1634375..1634827	-	453	WP_232046050.1	Rha family transcriptional regulator	-
C1P84_RS23730 (1634885)	1634885..1635343	-	459	WP_000961311.1	ash family protein	-
C1P84_RS08890 (1635356)	1635356..1635586	+	231	WP_005061113.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1P84_RS08895 (1635576)	1635576..1635884	+	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
C1P84_RS08900 (1635926)	1635926..1636102	-	177	WP_001252556.1	NinE family protein	-
C1P84_RS08905 (1636099)	1636099..1636509	-	411	WP_000814621.1	recombination protein NinB	-
C1P84_RS08910 (1636506)	1636506..1636784	-	279	WP_005061104.1	hypothetical protein	-
C1P84_RS08915 (1636859)	1636859..1637053	-	195	Protein_1698	hypothetical protein	-
C1P84_RS23735 (1638300)	1638300..1638374	+	75	Protein_1700	hypothetical protein	-
C1P84_RS08925 (1638385)	1638385..1638993	+	609	Protein_1701	PBSX family phage terminase large subunit	-
C1P84_RS08930 (1639084)	1639084..1640312	+	1229	WP_094081542.1	IS3-like element IS2 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH4.5	1597595..1651960	54365

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (5-70)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8665.12 Da
• Isoelectric Point: 10.5308

>T95817 WP_005061113.1 NZ_CP026776:1635356-1635586 [Shigella flexneri]
MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 231 bp

>T95817 NZ_CP026776:1635356-1635586 [Shigella flexneri]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT95817 WP_000147081.1 NZ_CP026776:1635576-1635884 [Shigella flexneri]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT95817 NZ_CP026776:1635576-1635884 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P7TJC1