Detailed information of TA system TA095798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4060811..4061438
• Replicon: chromosome
• Accession: NZ_CP026774
• Organism: Shigella dysenteriae strain ATCC 13313

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A828BKW8
• Locus tag: C1P61_RS22200
• Protein ID: WP_024258354.1
• Coordinates: 4061109..4061438 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q32DW6
• Locus tag: C1P61_RS22195
• Protein ID: WP_000147081.1
• Coordinates: 4060811..4061119 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P61_RS22165	4056808..4057149	+	342	Protein_4148	DUF2138 family protein	-
C1P61_RS22170	4057146..4057706	+	561	WP_134801719.1	DUF1175 domain-containing protein	-
C1P61_RS22175	4057764..4058768	-	1005	Protein_4150	tyrosine-type recombinase/integrase	-
C1P61_RS22180	4058902..4060133	+	1232	WP_134801668.1	IS3-like element IS600 family transposase	-
C1P61_RS22185	4060186..4060596	+	411	WP_000814621.1	recombination protein NinB	-
C1P61_RS22190	4060593..4060814	+	222	WP_001254231.1	NinE family protein	-
C1P61_RS22195	4060811..4061119	-	309	WP_000147081.1	helix-turn-helix transcriptional regulator	Antitoxin
C1P61_RS22200	4061109..4061438	-	330	WP_024258354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C1P61_RS22205	4061759..4062436	+	678	WP_000957540.1	phage antirepressor Ant	-
C1P61_RS22210	4062511..4063233	+	723	WP_001004003.1	DNA-binding protein	-
C1P61_RS22215	4063271..4063411	+	141	Protein_4158	HNH endonuclease	-
C1P61_RS22225	4063468..4064162	+	695	WP_134801589.1	IS1 family transposase	-
C1P61_RS22230	4064178..4064498	-	321	Protein_4160	ABC transporter substrate-binding protein ArtJ	-
C1P61_RS22235	4064789..4065457	-	669	WP_000464490.1	arginine ABC transporter permease ArtM	-
C1P61_RS22240	4065457..4066173	-	717	WP_001001693.1	arginine ABC transporter permease ArtQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4025946..4074401	48455
	-	inside	IScluster/Tn	-	-	4058902..4071327	12425

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12837.87 Da
• Isoelectric Point: 9.9966

>T95798 WP_024258354.1 NZ_CP026774:c4061438-4061109 [Shigella dysenteriae]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Nucleotide

• Length: 330 bp

>T95798 NZ_CP026774:c4061438-4061109 [Shigella dysenteriae]
ATGAACTACACTATCGAATACTACAGTGAAGATGTAAGGCTGGAAGTCGATCGGCTTCCATTGAGTATGCGTGCCCGATA
CCAACATCTCGTTGAACGTATGAAGATATATGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGACTTT
TCGAACTCAGAATTAAAGGCAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTG
CATAGTTTTGTAAAGAAAACGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11185.94 Da
• Isoelectric Point: 8.5840

>AT95798 WP_000147081.1 NZ_CP026774:c4061119-4060811 [Shigella dysenteriae]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT95798 NZ_CP026774:c4061119-4060811 [Shigella dysenteriae]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACCCTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A828BKW8

Antitoxin

• AlphaFold DB: A0A4P7TJC1