Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2012543..2013161 Replicon chromosome
Accession NZ_CP026768
Organism Shigella flexneri Y strain 93-3063

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C1P87_RS10655 Protein ID WP_001291435.1
Coordinates 2012943..2013161 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag C1P87_RS10650 Protein ID WP_000344797.1
Coordinates 2012543..2012917 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P87_RS10640 (2007632) 2007632..2008825 + 1194 WP_024260158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C1P87_RS10645 (2008848) 2008848..2011997 + 3150 WP_001132484.1 efflux RND transporter permease AcrB -
C1P87_RS10650 (2012543) 2012543..2012917 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
C1P87_RS10655 (2012943) 2012943..2013161 + 219 WP_001291435.1 HHA domain-containing protein Toxin
C1P87_RS10660 (2013333) 2013333..2013884 + 552 WP_000102577.1 maltose O-acetyltransferase -
C1P87_RS10665 (2014000) 2014000..2014470 + 471 WP_000136192.1 YlaC family protein -
C1P87_RS10670 (2014634) 2014634..2016184 + 1551 WP_005106783.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C1P87_RS10675 (2016226) 2016226..2016579 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C1P87_RS10685 (2016958) 2016958..2017269 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2017299..2018627 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T95770 WP_001291435.1 NZ_CP026768:2012943-2013161 [Shigella flexneri Y]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T95770 NZ_CP026768:2012943-2013161 [Shigella flexneri Y]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT95770 WP_000344797.1 NZ_CP026768:2012543-2012917 [Shigella flexneri Y]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT95770 NZ_CP026768:2012543-2012917 [Shigella flexneri Y]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References