Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 2372532..2372757 Replicon chromosome
Accession NZ_CP026765
Organism Shigella flexneri strain 04-3145

Toxin (Protein)


Gene name hokW Uniprot ID S1ELB5
Locus tag C1P85_RS12830 Protein ID WP_000813254.1
Coordinates 2372532..2372687 (-) Length 52 a.a.

Antitoxin (RNA)


Gene name sokW
Locus tag -
Coordinates 2372699..2372757 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P85_RS12765 2367965..2368662 + 698 WP_225620329.1 IS1 family transposase -
C1P85_RS12770 2368674..2368865 - 192 Protein_2398 helix-turn-helix domain-containing protein -
C1P85_RS12775 2368964..2369179 - 216 WP_000839572.1 class II holin family protein -
C1P85_RS12805 2369975..2370663 - 689 Protein_2400 bacteriophage antitermination protein Q -
C1P85_RS12810 2370660..2371025 - 366 WP_000140020.1 RusA family crossover junction endodeoxyribonuclease -
C1P85_RS12815 2371026..2372084 - 1059 WP_001265248.1 DUF968 domain-containing protein -
C1P85_RS12820 2372086..2372364 - 279 WP_011069426.1 hypothetical protein -
C1P85_RS12830 2372532..2372687 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD Toxin
- 2372699..2372757 + 59 - - Antitoxin
C1P85_RS12845 2373339..2373755 - 417 WP_005069274.1 hypothetical protein -
C1P85_RS12850 2373782..2373922 + 141 Protein_2406 DUF4224 domain-containing protein -
C1P85_RS12855 2373922..2374972 + 1051 Protein_2407 tyrosine-type recombinase/integrase -
C1P85_RS12865 2375183..2375980 + 798 WP_001325918.1 DgsA anti-repressor MtfA -
C1P85_RS12875 2376318..2377580 + 1263 Protein_2409 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH9.8 2350352..2409840 59488


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)


Sequences

Toxin        


Download         Length: 52 a.a.        Molecular weight: 5736.89 Da        Isoelectric Point: 6.1531

>T95729 WP_000813254.1 NZ_CP026765:c2372687-2372532 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Download         Length: 156 bp

>T95729 NZ_CP026765:c2372687-2372532 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 59 bp

>AT95729 NZ_CP026765:2372699-2372757 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TMW0

Antitoxin

Download structure file

References