Detailed information of TA system TA095718

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1713499..1713724
• Replicon: chromosome
• Accession: NZ_CP026765
• Organism: Shigella flexneri strain 04-3145

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C1P85_RS09140
• Protein ID: WP_000813254.1
• Coordinates: 1713569..1713724 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1713499..1713557 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1P85_RS24350	1709497..1709666	+	170	Protein_1694	hypothetical protein	-
C1P85_RS09105	1709812..1710558	+	747	WP_000788996.1	ATP-binding protein	-
C1P85_RS09110	1710573..1710995	+	423	WP_001118167.1	DUF977 family protein	-
C1P85_RS09115	1711053..1711409	+	357	WP_005048249.1	hypothetical protein	-
C1P85_RS09120	1711502..1711720	+	219	WP_000256998.1	DUF4014 family protein	-
C1P85_RS09125	1711722..1712087	+	366	WP_001229298.1	HNH endonuclease signature motif containing protein	-
C1P85_RS09130	1712084..1712749	+	666	WP_000208062.1	hypothetical protein	-
C1P85_RS09135	1712749..1713114	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	1713499..1713557	-	59	-	-	Antitoxin
C1P85_RS09140	1713569..1713724	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
C1P85_RS09155	1715061..1715660	+	600	WP_000940329.1	DUF1367 family protein	-
C1P85_RS09160	1715660..1715950	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
C1P85_RS09165	1715947..1716501	+	555	WP_000640143.1	DUF1133 family protein	-
C1P85_RS09170	1716654..1716827	+	174	WP_000504450.1	hypothetical protein	-
C1P85_RS09175	1716889..1718045	+	1157	WP_134797302.1	IS3-like element IS600 family transposase	-
C1P85_RS09180	1718038..1718598	+	561	WP_072075205.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	1704253..1766445	62192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T95718 WP_000813254.1 NZ_CP026765:1713569-1713724 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T95718 NZ_CP026765:1713569-1713724 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT95718 NZ_CP026765:c1713557-1713499 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin