Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 735221..735839 Replicon chromosome
Accession NZ_CP026765
Organism Shigella flexneri strain 04-3145

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C1P85_RS04020 Protein ID WP_001291435.1
Coordinates 735221..735439 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag C1P85_RS04025 Protein ID WP_000344797.1
Coordinates 735465..735839 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P85_RS03990 (731113) 731113..731424 - 312 WP_000409911.1 MGMT family protein -
C1P85_RS04000 (731803) 731803..732156 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C1P85_RS04005 (732198) 732198..733748 - 1551 WP_005106783.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C1P85_RS04010 (733912) 733912..734382 - 471 WP_000136192.1 YlaC family protein -
C1P85_RS04015 (734498) 734498..735049 - 552 WP_000102577.1 maltose O-acetyltransferase -
C1P85_RS04020 (735221) 735221..735439 - 219 WP_001291435.1 HHA domain-containing protein Toxin
C1P85_RS04025 (735465) 735465..735839 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
C1P85_RS04030 (736385) 736385..739534 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
C1P85_RS04035 (739557) 739557..740750 - 1194 WP_024260158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 729755..731083 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T95716 WP_001291435.1 NZ_CP026765:c735439-735221 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T95716 NZ_CP026765:c735439-735221 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT95716 WP_000344797.1 NZ_CP026765:c735839-735465 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT95716 NZ_CP026765:c735839-735465 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References