95714

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 622122..622816 Replicon chromosome
Accession NZ_CP026765
Organism Shigella flexneri strain 04-3145

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag C1P85_RS03420 Protein ID WP_001263491.1
Coordinates 622418..622816 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag C1P85_RS03415 Protein ID WP_000554759.1
Coordinates 622122..622415 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P85_RS03395 (617761) 617761..618258 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
C1P85_RS03400 (618475) 618475..620187 - 1713 Protein_595 flagellar biosynthesis protein FlhA -
C1P85_RS03405 (620159) 620159..620944 + 786 WP_000207561.1 putative lateral flagellar export/assembly protein LafU -
C1P85_RS03410 (621015) 621015..622070 + 1056 WP_128882050.1 DNA polymerase IV -
C1P85_RS03415 (622122) 622122..622415 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C1P85_RS03420 (622418) 622418..622816 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C1P85_RS03425 (622826) 622826..623278 + 453 WP_001059861.1 GNAT family N-acetyltransferase -
C1P85_RS03430 (623524) 623524..624567 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
C1P85_RS03435 (624629) 624629..625243 + 615 Protein_602 peptide chain release factor H -
C1P85_RS03440 (625300) 625300..626757 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
C1P85_RS03445 (627019) 627019..627477 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB 615202..663861 48659


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T95714 WP_001263491.1 NZ_CP026765:622418-622816 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T95714 NZ_CP026765:622418-622816 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT95714 WP_000554759.1 NZ_CP026765:622122-622415 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT95714 NZ_CP026765:622122-622415 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References