Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2897411..2898138 Replicon chromosome
Accession NZ_CP026762
Organism Shigella boydii strain NCTC 9850

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag C1P50_RS15595 Protein ID WP_000550189.1
Coordinates 2897824..2898138 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C1P50_RS15590 Protein ID WP_000560263.1
Coordinates 2897411..2897827 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P50_RS15575 2892571..2893617 + 1047 WP_000695473.1 alpha-glucosidase -
C1P50_RS15580 2894083..2894922 + 840 Protein_2952 alpha-glucosidase -
C1P50_RS15585 2895348..2897366 + 2019 WP_000121439.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
C1P50_RS15590 2897411..2897827 - 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
C1P50_RS15595 2897824..2898138 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
C1P50_RS15600 2898423..2899559 - 1137 WP_000018685.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
C1P50_RS15605 2899644..2900147 + 504 WP_001295542.1 M48 family metallopeptidase -
C1P50_RS15610 2900224..2900907 + 684 WP_001183037.1 vancomycin high temperature exclusion protein -
C1P50_RS15615 2900986..2901972 + 987 WP_004986115.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T95709 WP_000550189.1 NZ_CP026762:c2898138-2897824 [Shigella boydii]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T95709 NZ_CP026762:c2898138-2897824 [Shigella boydii]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT95709 WP_000560263.1 NZ_CP026762:c2897827-2897411 [Shigella boydii]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT95709 NZ_CP026762:c2897827-2897411 [Shigella boydii]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCCCCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTGCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References