Detailed information of TA system TA095654

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1276682..1277053
• Replicon: chromosome
• Accession: NZ_CP026755
• Organism: Escherichia coli strain AR_0077

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A1Y2ZGM7
• Locus tag: AM456_RS07450
• Protein ID: WP_042038331.1
• Coordinates: 1276859..1277053 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1276682..1276860 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM456_RS07425	1272637..1274010	+	1374	WP_094030107.1	ATP-dependent RNA helicase DbpA	-
AM456_RS07430	1274139..1275074	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
AM456_RS07435	1275126..1276361	-	1236	WP_023277532.1	site-specific integrase	-
AM456_RS07440	1276363..1276578	-	216	WP_000079604.1	excisionase XisR	-
AM456_RS07445	1276678..1276866	-	189	WP_001302840.1	DUF1187 family protein	-
-	1276682..1276860	+	179	NuclAT_0	-	Antitoxin
-	1276682..1276860	+	179	NuclAT_0	-	Antitoxin
-	1276682..1276860	+	179	NuclAT_0	-	Antitoxin
-	1276682..1276860	+	179	NuclAT_0	-	Antitoxin
AM456_RS07450	1276859..1277053	-	195	WP_042038331.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
AM456_RS07455	1277110..1277919	-	810	WP_000166319.1	recombination protein RecT	-
AM456_RS07460	1277912..1280512	-	2601	WP_104888914.1	exodeoxyribonuclease VIII	-
AM456_RS07465	1280614..1280889	-	276	WP_000632297.1	protein RacC	-
AM456_RS07470	1280964..1281134	-	171	WP_001352098.1	conserved protein, Rac prophage	-
AM456_RS07475	1281134..1281355	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1275126..1323747	48621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.94 Da
• Isoelectric Point: 8.9538

>T95654 WP_042038331.1 NZ_CP026755:c1277053-1276859 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKVISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T95654 NZ_CP026755:c1277053-1276859 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGTCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAACGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT95654 NZ_CP026755:1276682-1276860 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A1Y2ZGM7

Antitoxin