Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 95010..95704 Replicon chromosome
Accession NZ_CP026755
Organism Escherichia coli strain AR_0077

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag AM456_RS01155 Protein ID WP_001263491.1
Coordinates 95306..95704 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag AM456_RS01150 Protein ID WP_000554755.1
Coordinates 95010..95303 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM456_RS01125 90824..91291 + 468 WP_000725264.1 flagellar basal body-associated FliL family protein -
AM456_RS01130 91311..92030 + 720 WP_094029767.1 FliA/WhiG family RNA polymerase sigma factor -
AM456_RS01135 92043..92906 + 864 WP_001169518.1 flagellar motor stator protein MotA -
AM456_RS01140 92909..93832 + 924 WP_001232555.1 putative lateral flagellar export/assembly protein LafU -
AM456_RS01145 93903..94958 + 1056 WP_001226154.1 DNA polymerase IV -
AM456_RS01150 95010..95303 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AM456_RS01155 95306..95704 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AM456_RS01160 95714..96166 + 453 WP_075862062.1 GNAT family N-acetyltransferase -
AM456_RS01165 96357..97496 + 1140 WP_172837103.1 RNA ligase RtcB family protein -
AM456_RS01170 97493..98107 + 615 WP_094029765.1 peptide chain release factor H -
AM456_RS01175 98164..99621 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
AM456_RS01180 99882..100340 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T95644 WP_001263491.1 NZ_CP026755:95306-95704 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T95644 NZ_CP026755:95306-95704 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT95644 WP_000554755.1 NZ_CP026755:95010-95303 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT95644 NZ_CP026755:95010-95303 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References