Detailed information of TA system TA095643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 84554..85204
• Replicon: chromosome
• Accession: NZ_CP026755
• Organism: Escherichia coli strain AR_0077

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM456_RS01080
• Protein ID: WP_122992239.1
• Coordinates: 84554..84895 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: D3GSB3
• Locus tag: AM456_RS01085
• Protein ID: WP_000212551.1
• Coordinates: 84905..85204 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM456_RS01055	79559..80659	+	1101	WP_001181887.1	flagellar basal body P-ring protein FlgI	-
AM456_RS01060	80659..80958	+	300	WP_000867178.1	rod-binding protein	-
AM456_RS01065	81164..82540	+	1377	WP_077561333.1	flagellar hook-associated protein FlgK	-
AM456_RS01070	82555..83484	+	930	WP_094029773.1	flagellar hook-associated protein FlgL	-
AM456_RS01075	83501..84478	+	978	WP_094029772.1	flagellar hook-associated protein	-
AM456_RS01080	84554..84895	+	342	WP_122992239.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM456_RS01085	84905..85204	+	300	WP_000212551.1	XRE family transcriptional regulator	Antitoxin
AM456_RS01090	85262..86104	-	843	WP_122992238.1	winged helix-turn-helix domain-containing protein	-
AM456_RS01095	86591..87505	+	915	WP_000949085.1	lateral flagellin LafA	-
AM456_RS01105	87711..89027	+	1317	WP_094029769.1	flagellar filament capping protein FliD	-
AM456_RS01110	89050..89442	+	393	WP_000285319.1	flagellar export chaperone FliS	-
AM456_RS01115	89447..89758	+	312	WP_000415000.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13075.87 Da
• Isoelectric Point: 5.7441

>T95643 WP_122992239.1 NZ_CP026755:84554-84895 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRDFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T95643 NZ_CP026755:84554-84895 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGATTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10976.81 Da
• Isoelectric Point: 9.7665

>AT95643 WP_000212551.1 NZ_CP026755:84905-85204 [Escherichia coli]
MATLKELMAKQSPESQARIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT95643 NZ_CP026755:84905-85204 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGCGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: D3GSB3