Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 53251..53792 Replicon chromosome
Accession NZ_CP026755
Organism Escherichia coli strain AR_0077

Toxin (Protein)


Gene name yafQ Uniprot ID D8A3N8
Locus tag AM456_RS00900 Protein ID WP_000615978.1
Coordinates 53251..53529 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag AM456_RS00905 Protein ID WP_000729709.1
Coordinates 53532..53792 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM456_RS00885 50923..51501 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AM456_RS00890 51617..52384 + 768 WP_000333380.1 class II glutamine amidotransferase -
AM456_RS00895 52355..53095 - 741 WP_001225679.1 murein L,D-transpeptidase -
AM456_RS00900 53251..53529 - 279 WP_000615978.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AM456_RS00905 53532..53792 - 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AM456_RS00910 53978..54751 + 774 WP_122992241.1 C40 family peptidase -
AM456_RS00915 54927..55424 + 498 WP_000006244.1 transposase -
AM456_RS00920 55541..57634 - 2094 WP_094029782.1 flagellar biosynthesis protein FlhA -
AM456_RS00925 57618..58757 - 1140 WP_094029781.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.66 Da        Isoelectric Point: 10.0702

>T95642 WP_000615978.1 NZ_CP026755:c53529-53251 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLLLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T95642 NZ_CP026755:c53529-53251 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACTGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT95642 WP_000729709.1 NZ_CP026755:c53792-53532 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT95642 NZ_CP026755:c53792-53532 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACTATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G928

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References