Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1712577..1713174 Replicon chromosome
Accession NZ_CP026751
Organism Klebsiella pneumoniae strain AR_0066

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AM445_RS08510 Protein ID WP_004142563.1
Coordinates 1712857..1713174 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AM445_RS08505 Protein ID WP_004142561.1
Coordinates 1712577..1712864 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM445_RS08475 1708657..1708905 + 249 WP_002893037.1 DUF1158 domain-containing protein -
AM445_RS08480 1708923..1709264 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AM445_RS08485 1709295..1710410 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
AM445_RS08490 1710590..1711171 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
AM445_RS08495 1711171..1711539 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AM445_RS08500 1711659..1712309 + 651 Protein_1629 oxygen-insensitive NAD(P)H nitroreductase -
AM445_RS08505 1712577..1712864 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AM445_RS08510 1712857..1713174 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM445_RS08515 1713359..1714402 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
AM445_RS08525 1715069..1715935 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
AM445_RS08530 1716044..1717471 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T95619 WP_004142563.1 NZ_CP026751:c1713174-1712857 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T95619 NZ_CP026751:c1713174-1712857 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT95619 WP_004142561.1 NZ_CP026751:c1712864-1712577 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT95619 NZ_CP026751:c1712864-1712577 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References