Detailed information of TA system TA095599

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37371..37635
• Replicon: plasmid p266917_2_03
• Accession: NZ_CP026726
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: C4R22_RS30465
• Protein ID: WP_001387489.1
• Coordinates: 37483..37635 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	37371..37431 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS30450	33473..34543	-	1071	WP_011264050.1	IncI1-type conjugal transfer protein TrbB	-
C4R22_RS30455	34562..35770	-	1209	WP_011264049.1	IncI1-type conjugal transfer protein TrbA	-
-	35950..36007	-	58	NuclAT_1	-	-
-	35950..36007	-	58	NuclAT_1	-	-
-	35950..36007	-	58	NuclAT_1	-	-
-	35950..36007	-	58	NuclAT_1	-	-
C4R22_RS30460	36077..37168	-	1092	WP_069067420.1	protein finQ	-
-	37371..37431	-	61	NuclAT_0	-	Antitoxin
-	37371..37431	-	61	NuclAT_0	-	Antitoxin
-	37371..37431	-	61	NuclAT_0	-	Antitoxin
-	37371..37431	-	61	NuclAT_0	-	Antitoxin
C4R22_RS30465	37483..37635	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
C4R22_RS30470	37707..37958	-	252	WP_001291964.1	hypothetical protein	-
C4R22_RS30475	38258..38554	+	297	WP_011264046.1	hypothetical protein	-
C4R22_RS31970	38619..38795	-	177	WP_001054897.1	hypothetical protein	-
C4R22_RS30480	39187..39396	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
C4R22_RS30485	39468..40130	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
C4R22_RS30490	40201..42369	-	2169	WP_023518847.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83010	83010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T95599 WP_001387489.1 NZ_CP026726:37483-37635 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T95599 NZ_CP026726:37483-37635 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT95599 NZ_CP026726:c37431-37371 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin