Detailed information of TA system TA095592

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82414..82840
• Replicon: plasmid p266917_2_01
• Accession: NZ_CP026724
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C4R22_RS29205
• Protein ID: WP_001312861.1
• Coordinates: 82414..82572 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	82616..82840 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS29155	77522..77749	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
C4R22_RS29160	77837..78529	-	693	WP_104858478.1	PAS domain-containing protein	-
C4R22_RS29165	78723..79106	-	384	WP_023063829.1	relaxosome protein TraM	-
C4R22_RS29170	79449..80039	+	591	WP_167575169.1	transglycosylase SLT domain-containing protein	-
C4R22_RS29180	80334..81155	-	822	WP_001234469.1	DUF945 domain-containing protein	-
C4R22_RS29185	81274..81561	-	288	WP_000107526.1	hypothetical protein	-
C4R22_RS31935	81862..82111	+	250	Protein_94	hypothetical protein	-
C4R22_RS29205	82414..82572	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	82616..82840	-	225	NuclAT_0	-	Antitoxin
-	82616..82840	-	225	NuclAT_0	-	Antitoxin
-	82616..82840	-	225	NuclAT_0	-	Antitoxin
-	82616..82840	-	225	NuclAT_0	-	Antitoxin
C4R22_RS31685	82652..82831	+	180	WP_001309233.1	hypothetical protein	-
C4R22_RS29210	82852..83571	-	720	WP_001403352.1	plasmid SOS inhibition protein A	-
C4R22_RS29215	83568..84002	-	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
C4R22_RS29220	84057..84359	-	303	Protein_99	hypothetical protein	-
C4R22_RS29225	84439..84636	+	198	Protein_100	helix-turn-helix domain-containing protein	-
C4R22_RS31690	84691..84849	+	159	Protein_101	transposase	-
C4R22_RS29235	84865..85562	-	698	Protein_102	IS1 family transposase	-
C4R22_RS29240	85671..86882	-	1212	WP_001390768.1	dispersin export-associated protein AatD	-
C4R22_RS29245	86894..87523	-	630	WP_000621743.1	dispersin export ABC transporter ATP-binding protein AatC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / aap/aspU / aggA / aggB / aggC / aggD	1..129627	129627
	-	flank	IS/Tn	-	-	84865..85368	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T95592 WP_001312861.1 NZ_CP026724:c82572-82414 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T95592 NZ_CP026724:c82572-82414 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT95592 NZ_CP026724:c82840-82616 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin