Detailed information of TA system TA095588

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45103..45334
• Replicon: plasmid p266917_2_01
• Accession: NZ_CP026724
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: C4R22_RS28975
• Protein ID: WP_023144756.1
• Coordinates: 45103..45237 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45283..45334 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS28925	40442..40924	-	483	WP_001402899.1	hypothetical protein	-
C4R22_RS28930	41041..41880	-	840	WP_001402900.1	3'-5' exonuclease	-
C4R22_RS28935	41926..42207	-	282	WP_001402901.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C4R22_RS28940	42204..42473	-	270	WP_000079941.1	hypothetical protein	-
C4R22_RS28955	43390..44247	-	858	WP_000130641.1	incFII family plasmid replication initiator RepA	-
C4R22_RS28960	44240..44314	-	75	WP_031943482.1	RepA leader peptide Tap	-
C4R22_RS31930	44311..44445	-	135	Protein_51	protein CopA/IncA	-
C4R22_RS28970	44552..44806	-	255	WP_089643063.1	replication regulatory protein RepA	-
C4R22_RS28975	45103..45237	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	45283..45334	+	52	NuclAT_1	-	Antitoxin
-	45283..45334	+	52	NuclAT_1	-	Antitoxin
-	45283..45334	+	52	NuclAT_1	-	Antitoxin
-	45283..45334	+	52	NuclAT_1	-	Antitoxin
C4R22_RS28980	45301..45587	-	287	Protein_54	DUF2726 domain-containing protein	-
C4R22_RS28990	46099..46311	-	213	WP_104858475.1	ANR family transcriptional regulator	-
C4R22_RS28995	46443..47003	-	561	WP_000139341.1	fertility inhibition protein FinO	-
C4R22_RS29000	47058..47804	-	747	WP_032188604.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / aap/aspU / aggA / aggB / aggC / aggD	1..129627	129627
	-	flank	IS/Tn	-	-	39260..40282	1022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T95588 WP_023144756.1 NZ_CP026724:c45237-45103 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T95588 NZ_CP026724:c45237-45103 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 52 bp

>AT95588 NZ_CP026724:45283-45334 [Escherichia coli]
TCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin