Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4068694..4069388 Replicon chromosome
Accession NZ_CP026723
Organism Escherichia coli strain 266917_2

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag C4R22_RS20870 Protein ID WP_001263491.1
Coordinates 4068990..4069388 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag C4R22_RS20865 Protein ID WP_000554755.1
Coordinates 4068694..4068987 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4R22_RS20840 4064315..4064671 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C4R22_RS20845 4064664..4064942 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C4R22_RS20850 4065047..4066759 - 1713 Protein_3819 flagellar biosynthesis protein FlhA -
C4R22_RS20855 4066731..4067516 + 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
C4R22_RS20860 4067587..4068642 + 1056 WP_001550655.1 DNA polymerase IV -
C4R22_RS20865 4068694..4068987 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C4R22_RS20870 4068990..4069388 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C4R22_RS20875 4069398..4069850 + 453 WP_019842500.1 GNAT family N-acetyltransferase -
C4R22_RS20880 4070096..4070302 + 207 Protein_3825 RtcB family protein -
C4R22_RS20885 4070298..4070650 + 353 Protein_3826 peptide chain release factor H -
C4R22_RS20890 4070707..4072164 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
C4R22_RS20895 4072425..4072883 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
C4R22_RS20900 4072975..4074219 + 1245 WP_000189541.1 esterase FrsA -
- 4073479..4073559 + 81 NuclAT_11 - -
- 4073479..4073559 + 81 NuclAT_11 - -
- 4073479..4073559 + 81 NuclAT_11 - -
- 4073479..4073559 + 81 NuclAT_11 - -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T95579 WP_001263491.1 NZ_CP026723:4068990-4069388 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T95579 NZ_CP026723:4068990-4069388 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT95579 WP_000554755.1 NZ_CP026723:4068694-4068987 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT95579 NZ_CP026723:4068694-4068987 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7


Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References