Detailed information of TA system TA095573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3232142..3232744
• Replicon: chromosome
• Accession: NZ_CP026723
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C4R22_RS16645
• Protein ID: WP_000897305.1
• Coordinates: 3232142..3232453 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4R22_RS16650
• Protein ID: WP_000356395.1
• Coordinates: 3232454..3232744 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS16620	3227184..3227621	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C4R22_RS16625	3227666..3228607	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C4R22_RS16630	3229023..3230837	+	1815	WP_001550352.1	hypothetical protein	-
C4R22_RS16640	3231005..3231913	-	909	WP_001550353.1	alpha/beta hydrolase	-
C4R22_RS16645	3232142..3232453	+	312	WP_000897305.1	hypothetical protein	Toxin
C4R22_RS16650	3232454..3232744	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
C4R22_RS16655	3232829..3233050	+	222	WP_001550354.1	hypothetical protein	-
C4R22_RS16660	3233102..3233380	+	279	WP_001315112.1	hypothetical protein	-
C4R22_RS16670	3233799..3234017	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C4R22_RS16675	3234236..3234478	+	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
C4R22_RS16680	3234660..3235589	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
C4R22_RS16685	3235586..3236221	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C4R22_RS16690	3236218..3237120	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T95573 WP_000897305.1 NZ_CP026723:3232142-3232453 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T95573 NZ_CP026723:3232142-3232453 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT95573 WP_000356395.1 NZ_CP026723:3232454-3232744 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT95573 NZ_CP026723:3232454-3232744 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7