Detailed information of TA system TA095567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1790494..1791221
• Replicon: chromosome
• Accession: NZ_CP026723
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: C4R22_RS09245
• Protein ID: WP_000547555.1
• Coordinates: 1790910..1791221 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C4R22_RS09240
• Protein ID: WP_000126294.1
• Coordinates: 1790494..1790913 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS09220	1785579..1786106	-	528	WP_001078777.1	electron transport protein HydN	-
C4R22_RS09225	1786255..1787265	-	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
C4R22_RS09230	1787525..1788982	+	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C4R22_RS09235	1788991..1790415	+	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
C4R22_RS09240	1790494..1790913	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C4R22_RS09245	1790910..1791221	-	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
C4R22_RS09250	1791388..1791858	-	471	WP_001551542.1	hydrogenase maturation peptidase HycI	-
C4R22_RS09255	1791851..1792261	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C4R22_RS09260	1792258..1793025	-	768	WP_104858335.1	formate hydrogenlyase subunit HycG	-
C4R22_RS09265	1793025..1793567	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
C4R22_RS09270	1793577..1795286	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T95567 WP_000547555.1 NZ_CP026723:c1791221-1790910 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T95567 NZ_CP026723:c1791221-1790910 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT95567 WP_000126294.1 NZ_CP026723:c1790913-1790494 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT95567 NZ_CP026723:c1790913-1790494 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2