Detailed information of TA system TA095552

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 123000..123371
• Replicon: chromosome
• Accession: NZ_CP026723
• Organism: Escherichia coli strain 266917_2

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: C4R22_RS00630
• Protein ID: WP_001317028.1
• Coordinates: 123177..123371 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	123000..123178 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4R22_RS00605	118955..120328	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
C4R22_RS00610	120457..121392	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C4R22_RS00615	121444..122679	-	1236	WP_001551035.1	site-specific integrase	-
C4R22_RS00620	122681..122896	-	216	WP_000079604.1	excisionase XisR	-
C4R22_RS00625	122996..123184	-	189	WP_001551036.1	DUF1187 family protein	-
-	123000..123178	+	179	NuclAT_0	-	Antitoxin
-	123000..123178	+	179	NuclAT_0	-	Antitoxin
-	123000..123178	+	179	NuclAT_0	-	Antitoxin
-	123000..123178	+	179	NuclAT_0	-	Antitoxin
C4R22_RS00630	123177..123371	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C4R22_RS00635	123428..124237	-	810	WP_001551037.1	recombination protein RecT	-
C4R22_RS00640	124230..126881	-	2652	WP_001551038.1	exodeoxyribonuclease VIII	-
C4R22_RS00645	126983..127258	-	276	WP_001314664.1	hypothetical protein	-
C4R22_RS00650	127333..127503	-	171	WP_001551041.1	phage protein	-
C4R22_RS00655	127503..127724	-	222	WP_000560221.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	107896..168857	60961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T95552 WP_001317028.1 NZ_CP026723:c123371-123177 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T95552 NZ_CP026723:c123371-123177 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT95552 NZ_CP026723:123000-123178 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin