Detailed information of TA system TA095547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3422124..3422851
• Replicon: chromosome
• Accession: NZ_CP026719
• Organism: Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM439_RS16900
• Protein ID: WP_049134723.1
• Coordinates: 3422540..3422851 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0Q1H0
• Locus tag: AM439_RS16895
• Protein ID: WP_045349893.1
• Coordinates: 3422124..3422543 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM439_RS16885 (AM439_16885)	3419954..3421075	-	1122	WP_049134721.1	YbdK family carboxylate-amine ligase	-
AM439_RS16890 (AM439_16890)	3421181..3422071	+	891	WP_104879818.1	oxidoreductase	-
AM439_RS16895 (AM439_16895)	3422124..3422543	-	420	WP_045349893.1	helix-turn-helix domain-containing protein	Antitoxin
AM439_RS16900 (AM439_16900)	3422540..3422851	-	312	WP_049134723.1	type II toxin-antitoxin system HigB family toxin	Toxin
AM439_RS25885	3423311..3424021	+	711	WP_164913538.1	DUF4145 domain-containing protein	-
AM439_RS16915 (AM439_16915)	3425398..3425853	-	456	WP_049134725.1	hypothetical protein	-
AM439_RS16920 (AM439_16920)	3426144..3427025	-	882	WP_003858850.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12532.38 Da
• Isoelectric Point: 9.9435

>T95547 WP_049134723.1 NZ_CP026719:c3422851-3422540 [Enterobacter cloacae complex sp.]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPLPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLTDYYRRTKE

Nucleotide

• Length: 312 bp

>T95547 NZ_CP026719:c3422851-3422540 [Enterobacter cloacae complex sp.]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTTACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGATC
TTCATCAAACACATCAGCACTCACGCAGACTATGACAAGCTCACGGACTACTACCGGAGAACGAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15375.45 Da
• Isoelectric Point: 4.7132

>AT95547 WP_045349893.1 NZ_CP026719:c3422543-3422124 [Enterobacter cloacae complex sp.]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENASPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT95547 NZ_CP026719:c3422543-3422124 [Enterobacter cloacae complex sp.]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAGCTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCCGATAACCCGCTGGTAGATATGCTCACTG
CCAAAATCGATAAGTATGAAAACGCGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGCATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGTGATTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTTAACGGACAGCGCACGTTAACGCTAGATCATATGCGAGCGCTGGCGAAGCGTTTCGGTTTGCCGGTGA
GCGCATTTGTGGGGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J0Q1H0