Detailed information of TA system TA095544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2166299..2167029
• Replicon: chromosome
• Accession: NZ_CP026719
• Organism: Enterobacter cloacae complex sp. strain AR_0060

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: AM439_RS10710
• Protein ID: WP_023302825.1
• Coordinates: 2166299..2166613 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM439_RS10715
• Protein ID: WP_128755637.1
• Coordinates: 2166610..2167029 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM439_RS10685 (AM439_10685)	2161965..2163050	+	1086	Protein_2019	cellulase family glycosylhydrolase	-
AM439_RS10690 (AM439_10690)	2162956..2164140	-	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
AM439_RS10695 (AM439_10695)	2164316..2165149	-	834	WP_049134768.1	DMT family transporter	-
AM439_RS10700 (AM439_10700)	2165212..2165658	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
AM439_RS10710 (AM439_10710)	2166299..2166613	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
AM439_RS10715 (AM439_10715)	2166610..2167029	+	420	WP_128755637.1	helix-turn-helix domain-containing protein	Antitoxin
AM439_RS10720 (AM439_10720)	2167176..2167274	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
AM439_RS10725 (AM439_10725)	2167381..2169069	+	1689	WP_015570114.1	acetolactate synthase large subunit	-
AM439_RS10730 (AM439_10730)	2169073..2169360	+	288	WP_015570113.1	acetolactate synthase small subunit	-
AM439_RS10735 (AM439_10735)	2169486..2170079	+	594	WP_003861054.1	transcriptional regulator UhpA	-
AM439_RS10740 (AM439_10740)	2170076..2171581	+	1506	WP_049134767.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T95544 WP_023302825.1 NZ_CP026719:2166299-2166613 [Enterobacter cloacae complex sp.]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T95544 NZ_CP026719:2166299-2166613 [Enterobacter cloacae complex sp.]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15513.22 Da
• Isoelectric Point: 6.4736

>AT95544 WP_128755637.1 NZ_CP026719:2166610-2167029 [Enterobacter cloacae complex sp.]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMKEPNSPLLDIVCARISRYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT95544 NZ_CP026719:2166610-2167029 [Enterobacter cloacae complex sp.]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAAAGAGCCCAATAGCCCCTTGCTGGATATTGTGT
GCGCCAGAATTAGCCGTTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2